[1]王月松,王蓁,张华玲,等.PI3K/Akt通路在子宫内膜腺癌上皮间质转化中作用[J].齐鲁医学杂志,2017,32(03):285-287,290.[doi:10.13362/j.qlyx.201703011]
 WANG Yuesong,WANG Zhen,ZHANG Hualing,et al.THE ROLE OF PI3K/AKT SIGNALING PATHWAY DURING EPITHELIAL-MESENCHYMAL TRANSITION IN ENDOMETRIAL ADENOCARCINOMA[J].Medical Journal of Qilu,2017,32(03):285-287,290.[doi:10.13362/j.qlyx.201703011]
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PI3K/Akt通路在子宫内膜腺癌上皮间质转化中作用()
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《齐鲁医学杂志》[ISSN:1008-0341/CN:37-1280/R]

卷:
第32卷
期数:
2017年03期
页码:
285-287,290
栏目:
出版日期:
2017-08-07

文章信息/Info

Title:
THE ROLE OF PI3K/AKT SIGNALING PATHWAY DURING EPITHELIAL-MESENCHYMAL TRANSITION IN ENDOMETRIAL ADENOCARCINOMA
文章编号:
1008-0341(2017)03-0285-04
作者:
王月松1王蓁1张华玲1解秀杨2王廷恒1王笋1
1 青岛大学附属医院妇科,山东 青岛 266003; 2 青岛市妇女儿童医院
Author(s):
WANG Yuesong WANG Zhen ZHANG Hualing XIE Xiuyang WANG Tingheng WANG Sun
Department of Gynecology, The Affiliated Hospital of Qingdao University, Qingdao 266003, China
关键词:
子宫内膜肿瘤上皮-间质转化波形蛋白免疫组织化学
Keywords:
endometrial neoplasmas epithelial-mesenchymal transition vimentin immunohistochemistry
分类号:
R737.33
DOI:
10.13362/j.qlyx.201703011
文献标志码:
A
摘要:
目的 探讨磷脂酰肌醇-3-激酶/蛋白激酶B(PI3K/Akt)通路在子宫内膜腺癌上皮间质转化(EMT)过程中的作用。
方法 采用免疫组化PV-6000法检测12例正常增殖期子宫内膜、16例子宫内膜非典型增生、32例子宫内膜癌组织中磷酸化Akt(p-Akt)、上皮-钙黏蛋白(E-cadherin)及波形蛋白(Vimentin)的表达水平,并分析三者间及与各项临床病理参数的关系。
结果 3组内膜组织中p-Akt、E-cadherin和Vimentin阳性表达率比较,差异有显著性(χ2=13.91~19.28,P<0.05)。且在子宫内膜癌组织中p-Akt蛋白的表达与肿瘤病理分期及淋巴结转移有关(P=0.041、0.047),与肌层浸润程度和组织学分级无关(P=0.676、0.070);E-cadherin蛋白的表达与病理分期、肌层浸润程度和淋巴结转移有关(P=0.008、0.029、0.026),与组织学分级无关(P=0.640);Vimentin蛋白的表达与病理分期及淋巴结转移有关(P=0.019、0.012),与肌层浸润程度和组织学分级无关(P=0.647、0.648)。子宫内膜癌组织中p-Akt与Vimentin的表达水平呈正相关(r=0.462,P<0.05),与E-cadherin的表达水平呈负相关(r=-0.567,P<0.05)。
结论 PI3K/Akt通路可能通过介导EMT参与子宫内膜癌的浸润与转移。
Abstract:
Objective  To examine the role of PI3K/Akt signaling pathway in the process of epithelial-mesenchymal transition (EMT) in endometrial adenocarcinoma.
Methods  The protein expression of p-Akt, E-cadherin, and vimentin in 12 normal endometrium tissues,16 endometrium hyperplasia tissues, and 32 endometrial adenocarcinoma tissues was determined by PV-6000 immunohistochemistry. The relationship between the three proteins and each clinicopathological parameter was analyzed.
Results  The rates of p-Akt, E-cadherin, and vimentin expression were significantly different between the three groups (χ2=13.91-19.28,P<0.05). The expression of p-Akt in endometrial carcinoma tissues was associated with pathological stage and lymph node metastasis (P=0.041 and 0.047, respectively), but not with the degree of myometrial invasion and histological grade (P=0.676 and 0.070, respectively). The expression of E-cadherin was associated with pathological stage, degree of myometrial invasion, and lymph node metastasis (P=0.008, 0.029, and 0.026, respectively), but not with histological grade (P=0.640). The expression of vimentin was associated with pathological stage and lymph node metastasis (P=0.019 and 0.012, respectively), but not with degree of myometrial invasion and histological grade (P=0.647 and 0.648, respectively). In addition, the expression of p-Akt was positively correlated with vimentin expression (r=0.462,P<0.05), and was negatively correlated with E-cadherin expression in endometrial carcinoma tissues (r=-0.567,P<0.05).
Conclusion  The PI3K/Akt signaling pathway may be involved in tumor invasion and metastasis through mediating the EMT of endometrial adenocarcinoma.
更新日期/Last Update: 2017-08-12