[1]司晓辉,孙攀兴,杜增利.沉默WISP3基因抑制子宫内膜癌细胞增殖及侵袭力[J].齐鲁医学杂志,2017,32(06):631-635.[doi:10.13362/j.qlyx.201706001]
 SI Xiaohui,SUN Panxing,DU Zengli.SILENCING THE WISP3 GENE INHIBITS PROLIFERATION AND INVASION OF ENDOMETRIAL CANCER CELLS[J].Medical Journal of Qilu,2017,32(06):631-635.[doi:10.13362/j.qlyx.201706001]
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沉默WISP3基因抑制子宫内膜癌细胞增殖及侵袭力()
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《齐鲁医学杂志》[ISSN:1008-0341/CN:37-1280/R]

卷:
第32卷
期数:
2017年06期
页码:
631-635
栏目:
出版日期:
2018-03-20

文章信息/Info

Title:
SILENCING THE WISP3 GENE INHIBITS PROLIFERATION AND INVASION OF ENDOMETRIAL CANCER CELLS
文章编号:
1008-0341(2017)06-0631-05
作者:
司晓辉孙攀兴杜增利
焦作煤业(集团)有限责任公司中央医院,河南 焦作 454000
Author(s):
SI Xiaohui SUN Panxing DU Zengli
Jiaozuo Coal Industry (Group) Co., Ltd. Central Hospital, Jiaozuo 454000, China
关键词:
子宫内膜肿瘤Wnt诱导分泌蛋白3细胞增殖肿瘤侵润
Keywords:
endometrial neoplasms Wnt induced secreted protein 3 cell proliferation neoplasm invasiveness
分类号:
R737.33
DOI:
10.13362/j.qlyx.201706001
文献标志码:
A
摘要:
目的 探讨小分子干扰RNA(siRNA)沉默Wnt诱导分泌蛋白3(WISP3)基因对子宫内膜癌HEC-1A细胞增殖、凋亡和侵袭能力的影响。
方法 培养子宫内膜癌HEC-1A细胞,将其随机分为siRNA-WISP3组、siRNA-对照序列组和空白对照组,应用实时荧光定量PCR技术检测3组细胞中WISP3基因表达,MTT法检测3组细胞转染后增殖能力,流式细胞术检测3组细胞凋亡情况,Transwell法检测3组细胞迁移和侵袭的能力。
结果siRNA-WISP3组细胞中WISP3 mRNA相对表达量显著低于siRNA-对照序列组和空白对照组,差异有显著性(F=95.134,P<0.01)。与siRNA-对照序列组和空白对照组相比,siRNA-WISP3组细胞24、48、72和96 h时细胞增殖能力显著降低,差异有统计学意义(F=23.684~93.472,P<0.05)。siRNA-WISP3组细胞凋亡率显著高于siRNA-对照序列组和空白对照组,差异有统计学意义(F=130.685,P<0.01)。与siRNA-对照序列组和空白对照组相比,siRNA-WISP3组迁移细胞数和侵袭细胞数均降低,差异均有统计学意义(F=13.914、16.632,P<0.05)。
结论 特异性下调子宫内膜癌HEC-1A细胞中WISP3基因表达可抑制细胞增殖、迁移和侵袭能力,加速细胞凋亡,有望成为子宫内膜癌基因治疗的潜在靶位。
Abstract:
Objective  To investigate the effect of silencing Wnt induced secreted protein 3 (WISP3) by small interfering RNA (siRNA) on the proliferation, apoptosis, and invasion of human endometrial cancer cell line HEC-1A.
Methods  The HEC-1A cells were cultured and randomly divided into siRNA-WISP3 group, siRNA-control sequence group, and blank control group. The expression of the WISP3 gene, cell proliferation after transfection, and apoptosis in the three groups were determined by quantitative real-time PCR, MTT assay, and flow cytometry, respectively. The migration and invasion of cells in the three groups were detected by the Transwell method.
Results  The siRNA-WISP3 group had significantly lower relative expression of WISP3 mRNA than the siRNA-control sequence group and the blank control group (F=95.134,P<0.01). Compared with the siRNA-control sequence group and the blank control group, the siRNA-WISP3 group had reduced cell proliferation at 24, 48, 72, and 96 h (F=23.684-93.472,P<0.05). The apoptosis rate was significantly higher in the siRNA-WISP3 group than in the siRNA-control sequence group or the blank control group (F=130.685,P<0.01). Compared with the siRNA-control sequence group and the blank control group, the siRNA-WISP3 group had reduced numbers of migrating cells and invasive cells (F=13.914,16.632;P<0.05).
Conclusion  Specific knockdown of the WISP3 gene in HEC-1A cells might inhibit the proliferation, migration, and invasion, and meanwhile promote apoptosis. It is a potential target for gene therapy against endometrial cancer.
更新日期/Last Update: 2018-03-24