[1]褚杰,纪新强,朱迎春?.HA与CD44对卵巢癌细胞Nanog基因表达影响[J].齐鲁医学杂志,2017,32(06):639-641.[doi:10.13362/j.qlyx.201706003]
 CHU Jie,JI Xinqiang,ZHU Yingchun.INFLUENCE OF HYALURONIC ACID AND CD44 ON NANOG GENE EXPRESSION IN OVARIAN CANCER CELLS[J].Medical Journal of Qilu,2017,32(06):639-641.[doi:10.13362/j.qlyx.201706003]
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HA与CD44对卵巢癌细胞Nanog基因表达影响()
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《齐鲁医学杂志》[ISSN:1008-0341/CN:37-1280/R]

卷:
第32卷
期数:
2017年06期
页码:
639-641
栏目:
出版日期:
2018-03-20

文章信息/Info

Title:
INFLUENCE OF HYALURONIC ACID AND CD44 ON NANOG GENE EXPRESSION IN OVARIAN CANCER CELLS
文章编号:
1008-0341(2017)06-0639-03
作者:
褚杰纪新强朱迎春?
青岛大学附属医院妇科,山东 青岛 266003
Author(s):
CHU Jie JI Xinqiang ZHU Yingchun
Department of Gynecology, The Affiliated Hospital of Qingdao University, Qingdao 266003, China
关键词:
卵巢肿瘤基因Nanog透明质酸抗原CD44基因表达调控肿瘤
Keywords:
ovarian neoplasms genes Nanog hyaluronic acid antigens CD44 gene expression regulation neoplastic
分类号:
R737.31;R730.2
DOI:
10.13362/j.qlyx.201706003
文献标志码:
A
摘要:
目的 探讨透明质酸(HA)与CD44对卵巢癌细胞Nanog基因表达的影响。
方法 应用RT-PCR、序列测定等方法检测SKOV-3细胞Nanog基因的表达。在SKOV-3细胞培养液中分别加入HA、抗CD44抗体和HA,应用Western-blot方法检测细胞及细胞核内Nanog蛋白的表达。
结果 SKOV-3细胞中存在Nanog基因。SKOV-3细胞培养液中加入HA后Nanog蛋白表达增加,加入抗CD44抗体和HA后Nanog蛋白表达减少,差异均有显著意义(F=1 419.356、1 181.229,P<0.05)。
结论 HA、CD44可促进卵巢癌细胞Nanog基因表达,可能在卵巢癌细胞生长、侵袭和转移过程中发挥重要作用。
Abstract:
Objective  To investigate the influence of hyaluronic acid (HA) and CD44 on Nanog gene expression in ovarian cancer cells.
Methods  RT-PCR and sequencing were performed to measure the expression of Nanog gene in SKOV-3 cells. HA or anti-CD44 antibody+HA was added to the cell culture medium of SKOV-3 cells, and Western blot was used to measure the protein expression of Nanog in cells and cell nuclei.
Results  The Nanog gene was detected in SKOV-3 cells. There was a significant increase in the protein expression of Nanog after HA was added to the cell culture medium of SKOV-3 cells (F=1 419.356,P<0.05), while there was a significant reduction in the protein expression of Nanog after the addition of anti-CD44 antibody and HA (F=1 181.229,P<0.05).
Conclusion  HA and CD44 can promote the expression of the Nanog gene in ovarian cancer cells and thus may play an important role in the growth, invasion, and metastasis of ovarian cancer cells.
更新日期/Last Update: 2018-03-24