[1]王淑霞,于丽,何信佳,等.As2O3对人子宫肉瘤裸鼠移植瘤抑制作用及其机制[J].齐鲁医学杂志,2017,32(06):656-659.[doi:10.13362/j.qlyx.201706008]
 WANG Shuxia,YU Li,HE Xinjia,et al.INHIBITORY EFFECT AND MECHANISM OF ARSENIC TRIOXIDE AGAINST HUMAN UTERINE SARCOMA TRANSPLANTED INTO NUDE MICE[J].Medical Journal of Qilu,2017,32(06):656-659.[doi:10.13362/j.qlyx.201706008]
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As2O3对人子宫肉瘤裸鼠移植瘤抑制作用及其机制()
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《齐鲁医学杂志》[ISSN:1008-0341/CN:37-1280/R]

卷:
第32卷
期数:
2017年06期
页码:
656-659
栏目:
出版日期:
2018-03-20

文章信息/Info

Title:
INHIBITORY EFFECT AND MECHANISM OF ARSENIC TRIOXIDE AGAINST HUMAN UTERINE SARCOMA TRANSPLANTED INTO NUDE MICE
文章编号:
1008-0341(2017)06-0656-04
作者:
王淑霞于丽何信佳王海冀张业玲赵园园
青岛大学附属医院肿瘤科,山东 青岛 266003
Author(s):
WANG Shuxia YU Li HE Xinjia WANG Haiji ZHANG Yeling ZHAO Yuanyuan
Department of Oncology, The Affiliated Hospital of Qingdao University, Qingdao 266003, China
关键词:
三氧化二砷子宫肿瘤细胞凋亡
Keywords:
arsenic trioxide uterine neoplasms apoptosis
分类号:
R737.33
DOI:
10.13362/j.qlyx.201706008
文献标志码:
A
摘要:
目的 探讨三氧化二砷(As2O3)对人子宫肉瘤裸鼠移植瘤的抑制作用及其机制。
方法 裸鼠40只,建立子宫肉瘤皮下移植瘤模型,随机分为As2O3低、中、高剂量组及生理盐水(NS)组、异环磷酰胺(IFO)组5组,每天将相应剂量(1.0、2.5、5.0 mg/kg)As2O3、NS及IFO腹腔注射入裸鼠体内,连用10 d。停药后处死裸鼠,取肿瘤组织称瘤质量,比较各组抑瘤率;同时检测各组肿瘤组织细胞凋亡率及半胱氨酸天冬氨酸蛋白水解酶(caspase-3)与磷酸化细胞外信号调节激酶(p-ERK)基因的表达。
结果 As2O3各剂量组裸鼠一般情况可,与NS组裸鼠差别不大,IFO组裸鼠一般状态不佳。As2O3各剂量组及IFO组瘤质量均小于NS组,As2O3高剂量组及IFO组瘤质量小于As2O3中、低剂量组,差异有显著性(F=108.368,P<0.05)。As2O3各剂量组及IFO组细胞凋亡率均高于NS组,As2O3各剂量组细胞凋亡率高于IFO组,As2O3高剂量组细胞凋亡率高于中、低剂量组,差异有显著性(F=778.240,P<0.05)。As2O3各剂量组细胞caspase-3基因表达高于NS组及IFO组,差异有显著性(F=30.670,P<0.05)。As2O3高剂量组细胞p-ERK基因的表达阳性率低于NS组,差异有显著性(Z=-2.063,P<0.05)。
结论 As2O3对人子宫肉瘤裸鼠皮下移植瘤细胞增殖有抑制作用,并可能通过上调细胞中caspase-3基因表达促进细胞凋亡,而通过下调p-ERK基因表达影响信号传导通路抑制肿瘤生长。
Abstract:
Objective  To investigate the inhibitory effect and mechanism of arsenic trioxide (As2O3) against human uterine sarcoma transplanted into nude mice.
Methods  Human uterine sarcoma was subcutaneously transplanted into 40 nude mice. Those mice were randomly divided into low-, medium-, and high-dose As2O3 groups, normal saline (NS) group, and ifosfamide (IFO) group. Those mice received intraperitoneal injection of different doses of As2O3, IFO, or NS for 10 consecutive days and were sacrificed after drug withdrawal. Tumor tissues were collected for the measurement of tumor weight and between-group comparison of tumor inhibition rate. The apoptosis rate and expression of caspase-3 and phosphorylated extracellular signal-regulated kinase (p-ERK) in tumor tissues were compared between different groups.
Results  As2O3-treated mice, similar to those in the NS group, had better health conditions than those in the IFO group. The As2O3 and IFO groups had lower tumor weight than the NS group. The high-dose As2O3 group and the IFO group had significantly lower tumor weight than the medium-dose As2O3 group and the low-dose As2O3 group (F=108.368,P<0.05). The As2O3 groups had the highest apoptosis rates, followed by the IFO group and the NS group; the high-dose As2O3 group had a significantly higher apoptosis rate than the medium-dose As2O3 group and the low-dose As2O3 group (F=778.240,P<0.05). The As2O3 groups had significantly higher gene expression of caspase-3 than the NS group and the IFO group (F=30.670,P<0.05). The high-dose As2O3 group had a significantly lower gene expression rate of p-ERK than the NS group (Z=-2.063,P<0.05).
Conclusion  As2O3 can inhibit the proliferation of human uterine sarcoma subcutaneously transplanted into nude mice. The inhibitory effect may result from enhanced apoptosis by upregulated caspase-3 gene expression and repressed tumor growth by downregulated p-ERK gene expression and signaling pathway.
更新日期/Last Update: 2018-03-24