[1]张陈彦,都治香,祁美玉,等.三磷酸腺苷二钠氯化镁对宫内窘迫胎鼠心肌保护作用[J].齐鲁医学杂志,2017,32(06):688-691.[doi:10.13362/j.qlyx.201706016]
 ZHANG Chenyan,DU Zhixiang,QI Meiyu,et al.PROTECTIVE EFFECT OF DISODIUM ADENOSINE TRIPHOSPHATE-MAGNESIUM CHLORIDE ON THE MYOCARDIUM OF FETAL RATS WITH INTRAUTERINE DISTRESS[J].Medical Journal of Qilu,2017,32(06):688-691.[doi:10.13362/j.qlyx.201706016]
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三磷酸腺苷二钠氯化镁对宫内窘迫胎鼠心肌保护作用()
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《齐鲁医学杂志》[ISSN:1008-0341/CN:37-1280/R]

卷:
第32卷
期数:
2017年06期
页码:
688-691
栏目:
出版日期:
2018-03-20

文章信息/Info

Title:
PROTECTIVE EFFECT OF DISODIUM ADENOSINE TRIPHOSPHATE-MAGNESIUM CHLORIDE ON THE MYOCARDIUM OF FETAL RATS WITH INTRAUTERINE DISTRESS
文章编号:
1008-0341(2017)06-0688-04
作者:
张陈彦1都治香2祁美玉1张铭娜1赵敏英1张立新1
1 石家庄市第一医院产科,河北 石家庄 050011; 2 河北医科大学第一医院妇产科
Author(s):
ZHANG Chenyan DU Zhixiang QI Meiyu ZHANG Mingna ZHAO Minying ZHANG Lixin
Department of Obstetrics, The First Hospital of Shijiazhuang, Shijiazhuang 050011, China
关键词:
三磷酸腺苷二钠氯化镁心肌再灌注损伤丙二醛大鼠Sprague-Dawley胚胎哺乳动物
Keywords:
triphosphate and magnesium chloride fetal rats myocardial myocardial reperfusion injury malondialdehyde rats Sprague-Dawley embryo mammalian
分类号:
R36-33
DOI:
10.13362/j.qlyx.201706016
文献标志码:
A
摘要:
目的 研究三磷酸腺苷二钠氯化镁对宫内窘迫胎鼠心肌组织自由基和病理组织学的影响,探讨三磷酸腺苷二钠氯化镁对宫内窘迫胎鼠心肌组织的保护作用。
方法 成年雌性Sprague-Dawley孕鼠15只,随机分为缺血低氧再灌注组(I/R组)、假手术组(Sham组)、三磷酸腺苷二钠氯化镁治疗组(T/M组),每组5只。于妊娠第19天以35 g/L水合氯醛(10 mL/kg)腹腔注射麻醉,应用无损伤动脉钳钳夹双侧子宫、卵巢血管20 min建立缺血低氧宫内窘迫模型。拆除动脉夹恢复血供,并逐层关腹。缺血前30 min,T/M组以三磷酸腺苷二钠氯化镁45 mg/kg腹腔注射,Sham组和I/R组均给予9 g/L氯化钠注射液(45 mg/kg)。Sham组只进行开关腹手术,不钳夹子宫卵巢动脉。各组均于手术后24 h计算胎鼠死亡率;每组随机取10只胎鼠心肌组织制备标本,检测心肌组织的丙二醛水平;行苏木精-伊红染色,高倍镜下观察胎鼠心肌组织病理改变。
结果 I/R组胎鼠死亡率明显高于Sham组,T/M组胎鼠死亡率较I/R组显著降低,差异有显著性(χ2=12.938,P<0.05)。与Sham组比较,I/R组胎鼠心肌组织丙二醛含量显著增高,T/M组胎鼠心肌组织丙二醛活性显著降低,差异有显著性(F=174.9,P<0.05)。T/M组胎鼠心肌组织病理改变较I/R组轻。
结论 三磷酸腺苷二钠氯化镁能够减轻宫内窘迫胎鼠心肌损伤,对缺血低氧心肌有保护作用。
Abstract:
Objective  To study the effect of disodium adenosine triphosphate-magnesium chloride (T/M) on free radicals and histopathology of the myocardium in fetal rats with intrauterine distress, and to explore the protective effect of T/M on myocardial tissue in fetal rats with intrauterine distress.
Methods  Fifteen adult female Sprague-Dawley pregnant rats were randomly and equally divided into ischemia-reperfusion (I/R) group, sham-operation group, and T/M group. On day 19 of gestation, the rats were anesthetized by intraperitoneal injection of 35 g/L chloral hydrate (10 mL/kg). A model of ischemia/hypoxia/intrauterine distress was established by 20 min clamping of bilateral uterine and ovarian vessels using damage-free artery forceps. The artery forceps were removed to restore blood supply, and the abdomen was sutured layer by layer. At 30 min before ischemia, the T/M group received intraperitoneal injection of T/M at a dose of 45 mg/kg, while the sham-operation group and the I/R group were given 9 g/L sodium chloride solution at the same dose. The sham-operation group underwent abdominal surgery without clamping of the uterine and ovarian arteries. In all the groups, the rats were sacrificed at 24 h after surgery, and the mortality of fetal rats was calculated. For each group,10 fetal rats were randomly selected for myocardium specimen preparation. The level of malondialdehyde (MDA) in the myocardium was measured. HE staining was used to evaluate pathological changes in the myocardium.
Results  The mortality of fetal rats was significantly higher in the I/R group than in the sham-operation group, while the mortality of fetal rats was significantly lower in the T/M group than in the I/R group (χ2=12.938,P<0.05). Compared with the sham-operation group, the I/R group had a significantly increased level of MDA in the myocardium, while the T/M group had significantly lower MDA activity (F=174.9,P<0.05).
Conclusion  T/M can reduce myocardial injury in fetal rats with intrauterine distress and protect the myocardium from ischemia and hypoxia.
更新日期/Last Update: 2018-03-24